ABREU, Rayane Alexandre de.; CAMPOS, Magnólia Araújo.; SOUSA JUNIOR, Francisco Canindé de.; PADILHA, Carlos Eduardo de Araújo.; NÓBREGA, Franklin Ferreira de Farias.; VAZ, Michelle Rossana Ferreira.
Résumé:
Currentlywith advances inbiotechnology, in itsdifferent areas such aspharmaceuticals, food, cosmetics, agriculture, the development of newrecombinant proteinshas been an areaofgreat advances inlarge-scale productionfor differentpurposes. Because of their importance recentlyPR- 5 gene was isolatedand designated PaOLP, encodes a novel antifungal protein thatcancould be used inagriculturalbiotechnologystrategiesand/ ordosagein order to controlfungal diseases. In this context, the present work aimed to evaluatethe influenceof the compositionofthe culture medium in order
to optimizethe productionofrecombinant PaOLPofPhysalisangulatausing Escherichia coliM15as a host. Thegrowth protein PR-5 andexpression ofrecombinantprotein wasevaluated bydry weightmethodandby parametersfor thespreadsheetZajic. It proteinexpression PaOLPnotinterfere with can evaluating thekinetic concludedthat the the themetabolismof bacteriaanddemonstratedincreased growthand expression ina medium withoutcarbon source.
