Caracterização parcial à íons metálicos de uma protease com propriedades colagenolíticas extraída de vísceras digestivas de pescada-branca (Cynoscion leiarchus).

Abstract

The hake is a carnivorous species of fish, distributed along the northeastern coast, with great commercial importance. This study aimed characterize partly, the sensibility a protease with collagenolytic properties of C. leiarchus sensitivity to metal ions (Cu2+, K+, Zn2+ e Pb2+) in three different molarities (1, 5 and 10 mM). For this, we used 500g of intestinal viscera of C. leiarchus obtained through fishing colony in Ponta Verde, Maceió-AL, Brazil. The process of enzyme extraction was performed by maceration and homogenization. The sensitivity step was performed by direct exposure to metal ions. The results indicated that the ions in their respective molarities, showed different degrees of reduction of enzymatic activity. However, the enzyme was completely inactivated by the Pb2+ in a concentration of 10 mM. The results suggest that the sensitivity of the collagenolytic protease tested ions, information that can serve as a basis for future industrial applications.