BATISTA, I. S.; http://lattes.cnpq.br/1371773689261513; BATISTA, Ítalo da Silva.
Abstract:
Using plants for therapeutic purposes is a practice that accompanies mankind from the
beginning, but it is not because it is a natural product that can be used anyway, as many plants
may have unwanted toxic activity, so phytochemical studies are necessary. seeking greater
knowledge of the composition to be used, as well as expanding the therapeutic options.
Therefore, the present work aimed to carry out a phytochemical study of the plants Libidibia
ferrea and Schinopsis brasiliensis, popularly used for antioxidant, antimicrobial, anti-
inflammatory activities, among others, and among them the activity that aroused interest was
antimicrobial. For the phytochemical studies, hydroalcoholic extracts were produced in
different proportions for the two plants, which can be observed the presence of secondary
metabolites alkaloids, flavonoids, tannins and terpenes, found in different levels in the extracts
of the two species. For the analysis of the behavior of the constituents was used High
Performance Liquid Chromatography (HPLC), performing the standardization of the extracts
to obtain their chromatographic profile or fingerprint, seeking to observe the seasonal variation
of the constituents, which was found that the collection of Plants in different years led to
variations in their constituents even though they were collected at the same time of the year. To
confirm the antimicrobial activity, the two extracts were tested against five strains of genus
Candida and one strain of Cryptococcus sp. For this purpose, the extracts were microdiluted in
Saboraud Dextrose broth in 96-hole plates with a U-bottom, and the inoculum was later added
to the strains and the standard antifungal agent (amphotericin B) was used for control. After
that, the behavior of the microorganisms in the plates was verified, observing the change of
turbidity to identify their growth or not. Pointing antimicrobial potential of two strains tested
for Schinopsis brasiliensis.
