Produção de biofilme de amido de sorgo aditivado de extrato de própolis vermelha.

Abstract

Based on the need to find alternatives to reduce the environmental impact of the unrestrained use of plastic and its discarded waste, the present study aims to develop an active biofilm of 100% biological origin through the composition of modified starch from graniferous soybeans and lignocellulosic material with additives. of red propolis, being of great interest due to the availability of raw material and its low cost. The first stage of this work consisted of planting and obtaining agronomic data for the sorghum variety IPA-1011, with yield for green matter production 69.05±0.98 and dry matter production 19.88±0.47 in t. there is. The extracted starch showed a yield of 43% (b.s.). The second stage of the work was the oxidation modification of the starch with a 1.5% active chlorine solution, generating higher values for carbonyl (CO0) 068a±0.07 and carboxyl (COOH) 0.131±0.06. The ligonocellulosic material in its in natura characterization and after basic pre-treatment with the objective of solubilizing the lignin using a 23+3 central point planning, presented lignin for in natura bagasse 20.16±0.93 and lignin for pre-treated bagasse 3 .54±0.28. SEM analyzes for bagasse showed that after the pre-treatment step with NaOH, the fibers were more exposed and the outermost and roughest layer was removed, making the matrix more porous and thus providing the biomass with a better availability of cellulosic fibers. In the characterization of red propolis, the total phenol and flavonoid contents were 241.2 mg EAG/g and 36.1 mg EQ/g respectively. After the production of the films, values were found in its characterization that confirm that it is a good product for scale production: mechanical analyzes carried out on the two samples, denote differences in relation to tension and deformation in the rupture of the films, presenting a direct correlation between both analyses. The stress values in sample 03 were 0.145 mpa and strain at break 17.26%; The best results for water vapor solubility are found in the central point samples with the lowest solubility at 31.24%; As for the antibiogram analyzes against four strains of bacteria: Enterococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa, they had a good inhibitory result, since in the composition of the biofilm red propolis is added at a concentration of 2.5% as an additive, presenting greater inhibition in the antibiogram test against the strain of Bacillus subinitilis with an inhibition of 9.4 mm.