Estudo da ação dos extratos de Mimosa tenuiflora (Wild) Poiret e Piptadenia stipulacea (Benth) Ducke sobre cepas microbianas isoladas de mastite em búfalas.

Abstract

The buffaloes livestock has been penalized with various diseases, many of them similar to those found in cattle, such as mastitis, usually difficult to resolve leading to economic losses. Moreover, the indiscriminate use of broad-spectrum antimicrobial action has increased microbial resistance, making it increasingly difficult to treat. New alternative means have been sought in the treatment of microbial infections, in this context, this study aimed to evaluate a new alternative for control of mastitis in buffaloes with plants of the semi-arid in Paraiba state; Jurema preta (Mimosa tenuiflora (Wild) Poiret) and Jurema branca (Piptadenia stipulacea (Benth.) Ducke. We used shells of Jurema preta and Jurema branca collected of trees from farms in the de Campina Grande Federal University and Patos highway that connects to Campina Grande. Getting the ethanol extract was made with cold rotary evaporation. Were isolated and quantified the tannins M. tenuiflora and P. stipulacea and antimicrobial evaluation performed on these Staphylococcus aureus. The curved front of the bacterial extracts was assayed by Peyret method. Pharmacological tests were conducted acute (DL50) and skin sensitivity to ethanol extracts to evaluate their possible toxic effects. The antimicrobial activity was performed in vitro with strains of S. aureus and Staphylococcus spp. collected milk from buffaloes and cows with clinical and subclinical mastitis. The tests were performed in duplicates and the final result will be determined by the arithmetic mean of the inhibition halos. For the determination of antimicrobial activity in vivo, will be assessed the treatment of mammary glands from animals at different stages of lactation with mastitis detected by the CMT. The treatment was instituted for 5 days with administration intramammary of formulated product. Samples of milk from treated animals were collected on days 0, 1, 2, 3, 4, 5 after initiation of treatment, accompanied by the performance of CMT, culture, isolation and identification of microorganisms as previously mentioned, as well as counting the number colonies identified.