MELO, Roberto A.; RESENDE, Luciane Vilela.; MENEZES, Dimas.; BECK, Ana Paula Araújo.; COSTA, José Carlos da.; COUTINHO, Alisson Esdras.; NASCIMENTO, Ana Verônica Silva do.
Resumo:
With the development of new cultivars, a precise genetic
characterization is essential for improvement programs or for
cultivar registration and protection. Molecular markers have been
complementing the traditional morphological and agronomic
characterization techniques because they are virtually unlimited,
cover the whole genome and are not environmentally inluenced.
Genetic characterization constitutes the basis for studies involving
estimates of genetic similarity. Therefore, the objective of the present
study was to evaluate the genetic similarity between ten coriander
genotypes (nine cultivars and one line) using ISSR markers. The
cultivars used were: Americano, Asteca, Palmeira, Português, Santo,
Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-
9299. The genetic similarity between the cultivars was estimated
using 227 banded regions of ISSR molecular markers. The UBC
897 oligonucleotide generated the highest number of fragments (16),
resulting in a higher polymorphism. The results indicate that the
twenty-nine oligonucleotides chosen were satisfactory for detecting
polymorphism. Based on the grouping analysis determined from
the similarity data, there were two groups and two sub-groups. The
calculated similarity for the genotypes varied from 52 to 75%. The
lowest similarity was observed between Português and Verdão,
at 52%. The highest similarity was found between Português
and Palmeira, at 75%. The ISSR is eficient for identifying DNA
polymorphism in coriander.