TRUTA, Adriana Araújo Costa.; SOUZA, Ana Raquel Ribeiro e.; NASCIMENTO, Ana Verônica Silva do.; PEREIRA, Rita de Cássia.; BROMMONSCHENKEL, Sérgio Herminio.; CARVALHO, Murilo G.; ZERBINI JUNIOR, Francisco Murilo.
Abstract:
Identity and properties of potyvirus isolates obtained from
Capsicum spp.
Twenty isolates were obtained from Capsicum spp. plants
in the states of Minas Gerais, São Paulo, Espírito Santo and Rio de
Janeiro. The isolates were biologically characterized using a host
range assay and inoculation into a series of differential sweetpepper
(Capsicum annuum) cultivars. Two isolates from potato (Solanum
tuberosum) (PVYN-BR e PVYO-BR) were used as controls. The
isolates displayed a high degree of biological variability, although
they were all preliminarly identified as Potato virus Y (PVY). The
reaction in the differential cultivars enabled the classification of
the isolates as pathotypes 1 or 1.2 of PVY. The production of
antiserum was carried out using purified virions of one mild and
one severe isolate. Using these antisera in indirect ELISA, positive
ABSTRACT
reactions against all the isolates were observed. The antisera also
reacted against PVYN-BR and PVYO-BR, although the latter reacted
weakly. For molecular characterization, the polymerase (NIb) and
capsid protein (cp) genes and the 3’ non-translated region (3’NTR)
of biologically distinct isolates were cloned and sequenced.
Phylogenetic analysis confirmed the identity of six isolates as Pepper
yellow mosaic virus (PepYMV), a potyvirus recently described
infecting sweet pepper (Capsicum annuum) in Brazil. This result
suggests that PepYMV could be the predominant potyvirus causing
mosaic in Capsicum species in Brazil. Since PepYMV isolates have
host ranges similar to PVY (including the reaction in differential
cultivars), and the two viruses cross-react serologically, the
usefulness of the molecular analysis is demonstrated in the
identification of potyviruses from Capsicum spp.
