OLIVEIRA, V. P.; http://lattes.cnpq.br/8237164961960037; OLIVEIRA, Valter Pereira de.
Resumen:
The quantification of substances in human samples has great importance for the
diagnosis, monitoring and treatment for several situations that affect health and
physiological conditions of the population. Among the analytes to be quantified, you
have cyclosporine that is administered in transplanted patients for
immunosuppressive treatment. The drug quantification are currently done by
complex and expansive devices and is an important source of costs on public health
as Brazil has the Highest Attendance Transplant System of the World and subsidizes
95 % of the treatment , including surgical procedures , medication and monitoring
needed in post- transplant. Now a days, simple devices that do not require
instruments and laboratory facilities are being used for various types of diagnoses.
They are called rapid tests that use immunochromatography, as methodology. The
study had the scope to develop a material with chitosan films to carry similar
substances human blood and compare this capacity to the nitrocellulose with the
goal of establishing a possible replacement of nitrocellulose for chitosan in the
productions of a rapid test device, enabling a new field of application for chitosan as
a carrier material. The scalffolds had been developed by the preparation of a
chitosan 1% solution which was filtered, frozen and lyophilized. The scalffolds were
then neutralized, washed, dried and lyophilized again. The strips of nitrocellulose
registered with Anvisa were obtained in pharmacy, so samples were selected
randomly to be performed the same characterizations performed in scalffolds. The
characterization was performed using spectroscopy techniques in the Fourier
Transform Infrared Region (FTIR), Scanning Electron Microscopy (SEM) /Energy
Dispersive Spectroscopy X-ray (EDS), Wettability and Swelling. The infrared
spectra showed that the nitrocellulose and chitosan have functional groups
compatible with the features described in the literature. The micrographs and spectra
showed a composition compatible for characteristics of absorption and liquid carrying
due to the presence of polarity. The wettability tests have shown that the
nitrocellulose and chitosan have high hydrophilicity and swelling measurements
showed a high degree of nitrocellulose and chitosan swelling and its supporting
carrier material characteristics. The product developed has the potential feasibility for
use as carrier material in diagnostic devices.