SILVA, P. O. A.; http://lattes.cnpq.br/4592112396324265; SILVA, Pedro Odon Almeida.
Abstract:
Enterobacter cloacae, a bacterium found in the gastrointestinal tract and oral
cavity of healthy individuals, can represent an important opportunistic
microorganism that causes hospital-acquired infections, such as aspiration
pneumonia in patients admitted to Intensive Care Units. This bacterium is able
to form biofilms, secrete cytotoxins and is resistant to various antimicrobials. In
this sense, it is worth highlighting the growing search for natural products with
antimicrobial, antifungal and anti-adherent properties, such as essential oils, as
they are products of plant origin, low cost and easily accessible to the
population. Among the many essential oils with pharmacological properties,
Origanum vulgare stands out. The aim of this study was to analyze the
antibacterial and anti-adherent activity of Origanum vulgare essential oil against
Enterobacter cloacae. Methodological tests were carried out using broth
microdilution techniques in 96-well plates to determine the Minimum Inhibitory
Concentration (MIC) and Minimum Bactericidal Concentration (MBC), and the
inclined tube technique to determine the Minimum Inhibitory Concentration of
Adherence (MICA), in the presence of 5% sucrose. The essential oil of
Origanum vulgare was found to have an MIC of 128 µg/mL and CBM of 256
µg/mL against the Enterobacter cloacae strain. The MICA of the essential oil
and the positive control (0.12% chlorhexidine digluconate) were not found at the
concentrations tested. Therefore, it was possible to conclude that the essential
oil of Origanum vulgare had a high antimicrobial potential and was considered
bactericidal against Enterobacter cloacae, although there was no anti-adherent
activity at the concentrations tested and in the methodology applied.
