Avaliação da eficácia de fungos nematófagos na predação de larvas de nematódeos gastrintestinais de asininos submetidos a diferentes períodos de estocagem, sob baixa temperatura.

Abstract

The objective of this study was to evaluate the efficacy of pelleted formulations of Duddingtonia flagrans and Monacrosporium thaumasium sodium alginate, stored for two and five years, at temperatures between 2° and 8° C, on the predation of nematode infecting larvae after passage of the treatment gastrointestinal tract. Faecal samples were collected from 64 asininos for the counting of eggs per gram of feces (OPG). Asinins were divided into groups, each group containing eight animals, each animal received a single dose of 100g (20% fungal mycelium) of pellets along with commercial feed to facilitate ingestion. In the experiment with formulations of D. flagrans, the groups were divided as follows: GI - received D. flagrans pellets stored for five years; GII- received pellets of D. flagrans stored for two years; GIII - received newly produced D. flagrans pellets; and Control - received pellets without nematophagous fungi. In the experiment with formulations of M. thaumasium were divided: GI - received pellets of M. thaumasium stored for five years; GII - received pellets of M. thaumasium stored for two years; GIII - received pellets of M. thaumasium stored in newly produced pellets; and Control - received pellets without nematophagous fungi. It was observed that after passage of the pellets containing D. flangras (ACOO1) and M. thaumasium by the gastrointestinal tract of the asinines, regardless of pellet storage time, there was a significant larval reduction (p <0.01) up to 72 hours in assays A and B. Formulations containing D. flagrans showed larval reduction of up to 97% and formulations containing M. thaumasium larval reduction of up to 93.6%. It was concluded that the use of sodium alginate pellets containing D. flagrans and M. thaumasium stored up to five years were effective on the predation of infective nematode larvae after passage of the gastrointestinal tract of asinines.