Efeito da adição de sacarose e sulfato de cobre na descoloração do corante vermelho congo pelo fungo Lentinus Crinitus CCIBT 2611.

Abstract

This study aimed to evaluate the effect of the addition of sucrose and Copper Sulfate in decolorization of the dye Congo red by the fungus LentinuscrinitusCCIBt 2611. Dye decolorization was evaluated in the concentration of 20mg / L in different sucrose and copper sulfate concentrations in stationary liquid medium. The maximum discoloration rates were 84.7% in 96 hours of incubation at a concentration of 2.5% sucrose and copper sulfate (1mM). The micro-organism could keep its growth in the subjected dye concentration and tolerated its protracted exposure to different sucrose and copper sulfate concentrations, maintaining a biomass increase, reaching a maximum value of 2,31g / L in the concentration of 5% of sucrose. The pH ranged between 5 and 6.5, indicating optimal values for the fungus’ growth and discoloration. The enzyme activity showed that the microorganism was capable of producing the laccase enzyme, reaching the value of 87,46U / ml at a concentration of 5% sucrose. Based on all exposed above, LentinuscrinitusCCIBt 2611 has a high potential for azo dyes degradation, such as Congo Red, allowing the use of promising method for treating wastewater in the decolorization.