Produção de enzimas invertase por Aspergillus niger em fermentação em estado sólido.

Abstract

The invertase or β-D-fructofuranosidase is the main enzyme used in industry for the hydrolysis of sucrose and for obtaining invert sugar syrup. One of the methods used for invertase production is by solid-state fermentation (SSF), therefore, this work aims the production and evaluation of the enzyme invertase activity synthesized by the filamentous fungus Aspergillus niger IOC 4003, by solid state fermentation. The inoculum used in the process was a solution of water with potato and glucose, and the substrate used for the SSF was the passion fruit shell powder. Fermentation occurred in flasks containing the culture medium inoculated with the chosen substrate, where the whole process was maintained under incubation at 28 °C. The fermentation period was seven days, every 24 hours a sample of the fermentation broth was separated from the other samples, then the analysis of the broth was carried. Were determined invertase activity levels during the fermentation, the percentage humidity of the substrate, the variation of pH in the fermented broth, substrate consumption, and the concentration of proteins in the enzymatics samples by the Bradford method. The invertase production by Aspergillus niger IOC 4003 was satisfactory for solid-state fermentation. It can also be noted that the invertase activity levels were increasing during fermentation, and these activity levels were directly proportional to the quantities of proteins found in the enzymatic extract for each day of fermentation. The pH values remained at normal levels for the growth of fungus, which enabled the production of the enzyme. The production of enzymes by solid state fermentation is an affordable alternative in terms of sustainability and economic viability, turning organic waste into the desired product.