DIAS, E. C.; http://lattes.cnpq.br/4043659180983905; DIAS, Emanuele Cardoso.
Abstract:
Invertase can be known also as β-fructofuranosidase D, and hydrolase, that can be detected in
a wide variety of eukaryote and prokaryote organism. In the industry, it is the main enzyme
used in the hydrolysis of sucrose, and also in obtaining the invert sugar syrup. For this
enzyme production, the most widely way of fermentation in scientific production is the solid
state fermentation. This study aimed to analyze the performance of the SSF in the invertase
enzyme production, using the filamentous fungus Aspergillus niger IOC 4003 as an agent,
using two waste (bran and rice bran mesquite) as a substrate. The fermentation took place in
250 ml Erlenmeyer flasks containing the inoculated medium with the chosen substrate, where
the whole process is kept under incubation at room temperature. As regards the period of the
whole process was seven days. For every 24 hours were collected a erlenmeyer flask
containing the fermentation of each substrate. The extracted broth from each flask by vacuum
filtration had each pH, substrate consumption, enzymatic activity, percent moisture and
amount of crude protein analyzed. Cell growth was analyzed by dry weight, which took the
difference of the weights eppendorf tubes containing the dried biomass and sterilized empty
eppendorf tubes dried in an oven. According to this, the Aspergillus niger invertase IOC 4003
production was satisfactory by using of the solid state fermentation and the chosen residues. It
was observed that in both fermentations, the invertase activity levels increased during the
established period and shown to be directly proportional to the cell growth and productivity.
Since the Bradford protein quantification levels shown to be consistent even with a
fluctuation in fermentation using mesquite. The pH values maintained an acid character,
allowing a good performance of the fungus. In this way, the invertase production by solid
state fermentation provides a better more affordable viability, adding value to the waste that
would be discarded, and strengthening the sustainability and economic viability of the enzyme
production process.