Bioprospecção de tanases produzidas por fungos filamentosos da caatinga.

Abstract

The enzymes of microbial origin have many characteristics that promotes its use in several existing biotechnological processes, being relevant the advantages of enzymatic conversions in different industrial and environmental processes. Its production, in most cases, still economically costly, requiring selection of new microrganisms with high potential in the production of high value-added substances. The tannase (EC 3.1.1.20) is an enzyme able of hydrolyzing hydrolysable tannin´s ester and depsidic bonds, obtaining as products glucose and gallic acid or ellagic acid, gallic acid is an important substrate for the pharmaceutical and chemical industries. The Solid State Fermentation (SSF) is a great alternative for the production of this enzyme. The objective of this study was to select fungi strains from Caatinga Biome possibly producer of tanase. The selection of these strains was performed using as the single carbon source, 1% tannic acid. To determine the enzymatic activity of these species were tested in submerged fermentation incubated in shaker, for 144 hours, at 40° C in Mineral Medium containing: KH2PO4, MgSO4.7H2O, NaCl, (NH4)2 SO4, Malt Extract, Tannic Acid and distilled H2O. Of the 21 species tested 90.4% formed round around the colonies. The species chosen as the best producers tannase were LAB 07, LAB 14, 15 LAB, LAB, LAB 17 and LAB 20. The best results were obtained for LAB 07 and LAB 15, presenting activity of 0.155 and 0.133 UI, respectively. This tanases production study shows the high biotechnological potential of fungi Caatinga.