Efeito comportamental da indução de dislipidemia com nova metologia em ratas wistar.

Abstract

Dyslipidemias are characterized as a rise in plasma levels of total cholesterol (TC), low density lipoprotein (LDL) and triglycerides (TG), as well as by the reduction of high density lipoprotein (HDL) concentrations. Thus, the objective of this work was to evaluate if the use of egg yolk is capable of producing a similar effect to pure cholesterol in the induction of dyslipidemia and if there is any influence on the behavior of the animals after this process. For this, 19 animals were divided into 2 groups: EXP (experimental group) n = 9 and CON (control group) n = 10. The research was performed by inducing dyslipidemia for 14 days by the gavage method. To evaluate the effect of the emulsion on the anxiety of the animals, the tests of the open field and the one of the labyrinth in raised cross were done. The results were statistically analyzed using the Student's t-test and were applied in the Graph Pad prism program, being considered significant when they presented p <0.05. In the open field test, the experimental group had a lower number of defecations compared to the control group. The parameters of ambulation, rearing and grooming were not significantly different. Regarding the high cross-maze test, the experimental group decreased the number of entries as well as the length of stay in the open arms in relation to the control. Regarding the length of stay in the closed arms, the experimental group remained longer in the closed arms, but there were no significant differences in the frequency of entries. In the parameter that evaluates the dwell time in the central area, the experimental group remained less time in the central area of the apparatus compared to the control group. Regarding the head dives, the experimental group showed a decrease in the head dip when compared to the control. Therefore, such results demonstrate that the induction of dyslipidemia was able to provoke an anxiogenic effect contributing to the increase in the levels of anxiety in the animals.