CARVALHO, Ester Pereira de.
Resumen:
Studies have shown that diet has a great effect on the modulation of the intestinal
microbiota, contributing to the balance between microorganisms and host.
Modulation of the intestinal microbiota and immune response indicate a probable
application of probiotic products in the treatment of Inflammatory Bowel Diseases as
well as of the systemic diseases that accompany the inflammatory process, diseases
that act directly on the alteration of the lipid and glycemic profile. This study aimed to
evaluate the impact of administration of a rennet-like goat cheese added to
Lactobacillus rhamnosus EM1107 on colonic damage and biochemical parameters of
rats with induced colitis. Thirty-two rats of the winstar strain were divided into 4
groups: negative control (CN), colitic control (CC), probiotic cheese (QP) and
sulfasalazine (SZ), all receiving water and ad libtum ration. The QP group received
via gavage 2.5 mL of a solution containing 1 g of curd goat type added with
Lactobacillus rhamnosus EM1107 (10-7 UFC / g). After 15 days of pre-treatment, the
animals of the CC, QP and SZ groups were anesthetized for induction of colitis by
means of a rectal catheter, in which 0.25 mL of 30 mg DNBS (dinitrobenzene sulfonic
acid) solution was injected. On the fifth day after induction of colitis, the animals were
anesthetized and sacrificed by cardiac puncture, with collection of serum samples for
glucose, total cholesterol, HDL-cholesterol, triglycerides and LDL-cholesterol. The
colón was collected for macroscopic assessment of colonic damage. As for the
colonic damage score, a reduction was observed in the QP and SZ groups when
compared with the CC. The animals receiving treatment (QP and SZ) presented
lower values of weight and weight / length relation of intestinal colon, compared to
the others, demonstrating a reduction in the macroscopic damage of the colitic
lesion. Regarding the biochemical parameters, it was observed that the treatment
with the probiotic product caused a reduction in the glycemic levels being in amounts
similar to the animals that did not have injury. This shows the hypoglycemic effect.
The same happened as the lipid profile, with reduction of serum cholesterol, LDL and
triglycerides and increase of HDL. Goat's milk added from probiotic culture showed to
have intestinal anti-inflammatory activity, hypolipidic and hypoglycemic effect when
administered as a pretreatment in colitic animals.
