Aplicação de diferentes argilas na síntese do 6 – O – acetil – 1, 2; 3, 4 – Di – O – Isopropilideno – Alfa – D – Galactopiranose e avaliação toxicológica frente às larvas da Artemia Salina Leach.

Abstract

Carbohydrates are the most abundant polyfunctional macromolecules in nature, and have numerous medicinal, biological and technological applications. This study aims to study the reaction conditions for the synthesis of 6-O-acetyl-1,2: 3,4-di-O- isopropylidene--D- Galactopyranose using different types of clays and to evaluate their toxicity to the bioassay with Artemia saline Leach. In this sense, 1,2: 3,4-di-O- isopropylidene--D-galactopyranose was first synthesized from D-galactose in acid medium, yielding 83% yield. Then, the catalytic potential of different clays employed in the acetylation reaction of 1,2: 3,4-di-O-isopropylidene--D-galactopyranose was evaluated, where the best results were obtained using montmorillonite K-10 with The 95% yield, in addition, was carried out the study of the variation of the concentration of the montmorillonite K-10, where using 100% m / m of the clay, the reaction occurred in only 7.5 minutes with a yield of 95%. The reuse of the clay was studied in successive reactions of acetylation, where the montmorillonite K-10 maintained its catalytic activity for 4 reactional cycles. 1,2,3,4-di-O-isopropylidene--D- galactopyranose acetylation reaction, the solvents dichloromethane and ethyl acetate, where yields were 80% and 96%, respectively. Finally, toxicological bioassays were carried out against Artemia Salina Leach with 1,2: 3,4-di-O- isopropylidene--D-galactopyranose and 6-O-acetyl-1,2,4,4-di -O-isopropylidene-- D-galactopyranose, where they were nontoxic comparing the data in the literature. In this way, the reuse of clay was possible, the synthesized compounds were characterized and proved to be non-toxic, thus promising for possible biological applications.