Desenvolvimento e avaliação de arcabouços tridimensionais de quitosana-gelatina para regeneração tecidual de pele.

Abstract

The skin is the organ of the body more susceptible to injury at various levels. It is the largest organ of the body, work as coating of the body and acts as a protection against the inconvenience of the external environment. Among the various types of injury to the skin is exposed, may be mentioned burns, which one have various types and degrees of complexity, and various reasons, thereafter each one needs individual treatments.The asepsis and the use of bandages are procedures commonly used to prevent loss of body fluids and control of infection, these areamong the most important requirements for effective regeneration of the affected area. The chitosan and gelatin are biopolymers known for their biocompatibility and biodegradability, their also have certain characteristics which together may contribute to obtain materials more efficient for use in this in dressings. Thus, this study aims to develop three-dimensional scaffolds of chitosan by the technique of freeze-drying and subsequent surface coating with gelatin. The research was divided into two methods in order to facilitate discussion and to better respond to obtain desired application. For this purpose, various characterization techniques were performed in both in the first stage, asin the secondaims to discuss and development satisfactory search expectations for the future generation of a salable product.At the end were obtained interconnected porous structure with average pore diameters between 43.8um and 200pm, average swelling between 1.150% and the permeation rate to water vapor of 4,156 g/m2 day. Mechanical testing demonstrated that the frameworks produced elastic modulus on average of 8.1MPa. The results of cell viability of the scaffoldspresented percentage higher than 90%. The results demonstrate that the research objective was achieved, to improve the properties of chitosan scaffolds with gelatin, and indicate that this application for tissue regeneration of the skin has the potential to lower levels of complexity.