SOUSA, W. J. B.; http://lattes.cnpq.br/3417672972335953; SOUSA, Wladymyr Jefferson Bacalhau de.
Resumen:
Each year researchers have sought increasingly to develop or refine materials that
minimize health problems and promote or assist bone regeneration. The biomaterials
allow developing products that meet the needs of the body in order to aid in
reconstructive procedures and consequently improve the quality of life. Chitosan, due
to its characteristics of biodegradability, biocompatibility and biofunctionality, has
aroused the interest of researchers in order to obtain new materials and when
applied to biomedicine, becomes a biomaterial that encourages the incorporation of
other materials such as apatite which is present in bones and vitamin E which
maximizes the composite because it has the power to assist in tissue regeneration,
promoting the regeneration of bone defects. This study aimed to develop
biodegradable films of chitosan / calcium phosphate apatite-incorporated with
Vitamin E for use as biomaterials. In this study the composites of chitosan / apatite
(1, 3 and 5%) with and without vitamin E (15 mg proportion of film) were prepared
and characterized by X-ray diffraction (XRD), spectroscopy Transform Infrared
Region Fourier spectroscopy (FTIR), Contact Angle Wettability by, Analysis of
Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM),
Energy Dispersive Spectroscopy X-ray (EDS), optical microscopy (OM), Assay
Enzymatic Biodegradation , Assay and Cytotoxicity Determination of nitric oxide
production. The technique XRD showed that incorporation of vitamin E and semicrystalline
apatite alter the profile of chitosan membrane making it more crystalline.
With the FTIR technique was found that addition of vitamin E and apatite membranes
of chitosan did not alter the functional groups characteristic of chitosan. The analysis
by Contact Angle Wettability indicates hydrophilicity of the composites. The
chemicals present in the chitosan and vitamin E as well as the apatite composites
were detected by the technique of EDX. Through the techniques of OM and SEM, it
was possible to observe that the incorporation of apatite and vitamin E in the
chitosan membrane and also showed the presence of granular particles of varying
sizes and shapes. We conclude with the results of DSC that apatite did not alter the
thermal stability of chitosan unlike that of vitamin E increased the stability of the
composite and the same, unlike other elements, presented two endothermic peaks.
The biodegradation testing showed that the membrane is biodegradable especially in
the presence of lysozyme. The MTT assay and confirmed NO biocompatibility,
because the composites showed little or no cytotoxicity. Fundamentado by data
obtained from this study it can be concluded that the membrane of chitosan-vitamin E
with 1%, 3% and 5% apatite shows viability for use as a biomaterial in bone
regeneration.
