Produção, caracterização e aplicação do extrato bruto de L. crinitus na descoloração in vitro do corante vermelho congo.

Abstract

Inadequate disposal of toxic substances, such as dyes, is an environmental problem that aims to develop technologies capable of eliminating or eliminating the toxicity of substances that can be disposed of in the environment without ecosystems and human health. The use of microorganisms and / or enzymes of microbial origin has aroused interest for the removal of these dyes from the textile effluents. Among the microorganisms used in the degradation of recalcitrant xenobiotic compounds. An important enzyme of the ligninolytic complex produced by fungi is an enzyme that, unlike the potential for degradation of pollutant molecules, has been called the "ecofriendly" enzyme. (FES) using the enzyme laccase in fermentation in sustainable state (FES) using peanut the substrate. Quality-the-oil-oleo-oil-oleo-oil-oleo-calfry-oil-ui-cals (22), with 30 days, with 30 days, with 30 days adjusted substrate index to 70% (bu) and with addition of 2% soybean oil (m: v). The crude enzymatic extract gained the peak of production and was used for in vitro discoloration of Congo Red dye. In the discoloration of the dye, the effect of increasing the presence of CuSO4 was evaluated in a factorial design 22, after 24h of incubation. Peak laccase production was observed at 6 days of incubation. The laccase does not present crude extract of L. crinitus temperature and pH optimum of 50 ° C and 3,0, respectively. The enzyme was present in all the samples evaluated. The discoloration of Congo Red dye was influenced by the addition of CuSO 4. Results demonstrating the potential crude extract of L. crinitus to degrade the studied azo dye.