Identificação e caracterização microbiana em cadáveres sob as condições de laboratório.

Abstract

The use of corpses is indispensable for an undergraduate course in the health area. Practices performed in laboratories should follow regulations for the health and safety of students, teachers and employees . Even by formol, laboratory users are subject to the risk of contamination by pathogenic microorganisms. That may be present in anatomical tools, in the atmospheric environment and in corpses . For the s afety and health of users, this study aims to identify bacteria and fungus in the environment and in the tools of the Anatomy Laboratory of the Training Center of Teachers of the Feder al University of Campina Grande, Then, to present the potential of risks , the degree of resistance of bacteria to antibiotics and fungal diversity, to propose alternatives to improving the environmental conditions for the laboratory. The bacteria isolated were characte rized by gram positive and gram negative by the Gram method . Disk diffusion was used to reveal the sensitivity profile. The isolated filamentous fungi are of the genus: Aspergillus, Cladosporium, Epicoccum, Stemphylium and the yeast Candida albicans The data generated from daily observations, during one week, pre sented a considerable increase of the bacterial colony forming units The susceptibility profile of the gram positive bacteria presented greater sensitivity to the 10 tested antibiotics and the gram negative showed greater antimicrobial resistance. The imp ortance of knowing the sensitivity profile of bacteria is related to the prevention of possible infectious episodes when manipulating the tools, especially the ones with potentially resistant bacteria. In the identification of the fungi by coverslip cultiv ation, the predominance of Aspergillus was verified referring to allergic causes and respiratory infections and other pathogenicities that cause severe health problems to users. It is understood that the occurrence and knowledge of microbial agents in envi ronments with greater circulation of users are essential to the prevention and control of risk factors and health problems.