Estudo da toxicidade de Vitex gardneriana Shauer (Lamiaceae) em Artemia salina e sua eficácia sobre Haemonchus contornus Cobb, 1898 de ovinos.

Abstract

The objective of the research was to evaluate the toxic potential of Vitex gardneriana vitro on Artemia salina microcrustacean using hydroalcoholic extract of the leaves (EHF) with acute toxicity tests (LC50) at concentrations of 100, 500, 1,000, 10,000, 20,000, and 50,000 / mL. As positive control we used sodium hypochlorite (NaClO) to 1%. Was used as a negative control saline solution (pH 8-9) and 1% DMSO. The results showed lethality in 50% of the population metanaúplios microcrustacean concentration of 34,600 mg / mL, demonstrating their low toxic potential. To determine the toxic action of the hydroalcoholic extract of V. gardneriana on eggs and larvae of Haemonchus contortus was added 2.5mL of said EHF plant at a concentration of 34,600 mg / ml added at the monospecific fecal nematode cultures containing approximately 12,000 eggs. The results demonstrated that EHF allowed only 26.6% of the total 12,000 hatching eggs, corresponding to a therapeutic efficiency of 73.3%. For EHF assessment V. gardneriana on the parasite in sheep in vivo test, two doses were offered at concentrations of 34,600 / ml and 69.200 g / ml, where the lower dose showed an efficiency (49.6%) lower than the drug-treated group (63.3%), however for the higher dose showed an efficacy of EHF 63.33%, being equal to the group treated with the synthetic drug (ivermectin 1%), showing their possible use in additional alternative to control the parasite. During the study, the animals remained clinically healthy.