EULÁLIO, E. J. C.; http://lattes.cnpq.br/6826498016230269; EULÁLIO, Eliza Juliana da Costa.
Resumo:
INTRODUCTION to Diabetes Mellitus is a chronic disease which is increasing among
the Brazilians; it is directly related to the modern world risk factors. The main
complications of this disease are ulcers, hard treatment, slowed healing and the
ineffectiveness of some curatives. The advance in the field of biomaterials has
favored the development of products for this purpose, chitosan is a promising and
very relevant polymer, which may have its enhanced therapeutic action with the
incorporation of drugs, such as quercetin, a flavonoid with a proven anti -inflammatory
function. OBJECTIVE Developing chitosan membranes incorporated with quercetin,
for use in patients with epithelial ulcerations, in particular for use in diabetic wounds.
METHODOLOGY The present research was developed in Certbio (UFCG), it was
classified as experimental and it was produced 500 membranes obtained in the two
work phases: Exploratory and Experimental phases. Data were analyzed using
descriptive statistics, after the characterization tests: SEM, EDS, FTIR, XRD,
thermogravimetry (Tg), Analysis of Differential Scanning Calorimetry ( DSC) and
Cytotoxicity Assay. RESULTS The results of the exploration phase showed that
although there was the incorporation of quercetin in the polymer matrix of chitosan,
its contact with the basic solution (Sodium Hydroxide and Ammonium Hydroxide)
resulted in the formation of a salt, confirmed after observation of other tests , there
was also the formation of agglomerates due to the drug high concentration. It was
concluded that it showed no potential features for the applicability of the product in
diabetic wounds the results from experiment showed quercetin incorporated in the
chitosan matrix in both metolodogias (X and Y), but there was the crosslinking of
TPP (X), the drug was dispersed homogeneously, the diffraction showed crystallinity
change of membrane in the presence of quercetin, strengthening the interaction
between them. The analysis of TG and DSC showed that crosslinking in TPP
changed the thermal behavior of the membrane of chitosan / quercetin, increasing
the stability of the composite and que rcetin contributed discreetly to changes in heat
curves. The cytotoxicity assay showed that membranes with TPP have the possibility
to be used as a biomaterial. CONCLUSIONS It was concluded that the methodology
considered valid and effective for obtaining chitosan membranes / quercetin was X,
where there was crosslinking in TPP, with the potential to be tested in vivo and
contribute to tissue regeneration and combating inflammatory process present in
diabetic wounds. It is suggested that new tests are carried out in order to improve
some of the final characteristics of the product, however basic parameters of the
variables have already been established in this study.