Membranas porosas de quitosana/gelatina para liberação controlada de insulina.

Abstract

The study of drug controlled release system is constantly growing, it aims to improve and extend the control of drug administration. Oral insulin is a dream of patients and a challenge for scientists. For patients, it's not just pain relief applying multiple injections, but also the protection of beta cells of the pancreas. Chitosan, a biomaterial is considered non-toxic, non-allergenic, biodegradable, biofunctional, biocompatible and their biological activities include the antioxidant action, antimicrobial, analgesic, acceleration of wound healing, anti-inflammatory as well as being widely studied as polymer matrix systems controlled drug release. Gelatin turn is being widely used in the pharmaceutical field for the purpose of favoring the system swelling and consequently accelerate the release process. Thus, this study aimed to develop chitosan membranes, gelatin and insulin for use in controlled release system of drugs. The developed membranes were characterized by powder Diffraction X-ray (XRD), spectroscopy in the infrared region with a Fourier transform (FTIR) analysis, Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy X-ray (EDS) and Microscopy optical (MO). In XRD technique we found that what was no significant change in the crystallinity of the membranes. With FTIR technique found that gelatin, insulin and sodium tripolyphosphate did not affect the surface functional groups of chitosan, thereby maintaining the properties thereof. The EDS was possible to detect the characteristic chemical elements of the material. It was possible to see, through the techniques of OM and SEM, change in morphology of the membrane containing insulin, gelatin and sodium tripolyphosphate compared to pure chitosan. Based on the results it can be concluded that insulin was encapsulated by chitosan and the presence of the gelatin influence the size and shape of the pores of the membranes and neutralization with NaOH decreased the amount of agglomerates on the surfaces of scaffolds crosslinked with sodium tripolyphosphate.